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Scientist I, Protein Mass Spectrometry

PTC Therapeutics
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The Scientist I, Protein Mass Spectrometry will use cutting edge mass spectrometric techniques to identify new biological targets, verify compound engagement and ascertain pharmacodynamic effects across the PTC Therapeutics pipeline. The candidate will join a diverse team employing multi-omics approaches to interrogate systems level biological changes associated with genetic and/or disease phenotypes.
 
The Scientist I, Protein Mass Spectrometry is responsible for planning and performing scientific experiments that contribute to PTC’s research and drug discovery activities. The incumbent works cross-functionally with internal departments, and external resources as appropriate, as part of PTC’s discovery science project teams.  The Scientist I, Protein Mass Spectrometry supports adherence to relevant regulatory requirements and company Standard Operating Procedures (SOPs) as appropriate.

Job Description:
ESSENTIAL FUNCTIONS
Primary duties/responsibilities:

  • Develop SRM/PRM assays to quantitate protein targets in plasma, urine, CSF, and tissues.
  • Utilize both labeled (i.e. SILAC, TMT, etc.) and label-free methodologies for global proteomic analysis of in vitro systems.  
  • Use co-IP and/or XL-MS protocols to map interaction networks of therapeutic protein targets.
  • Process and analyze LC-MS data. Integrate results with other multi-omics datasets to visualize and track changes in a systems biology approach.
  • Use native MS with ion mobility to probe protein interactions with ligands, lipids, drugs, etc.
  • Presents findings internally to cross-functional teams.
  • May Participate in the development and writing of patent applications
  • Abides by all safety and regulatory guidelines.
  • Maintains a detailed laboratory notebook.
  • Performs other tasks as assigned
  • Interviews and contributes to the evaluation of candidates.
  • May provide scientific advice to research leadership on projects and areas of expertise.
  • Abides by all safety requirements.
  • Contributes to general lab needs such as:
Equipment maintenance.
Capital equipment purchases.
Troubleshoot
KNOWLEDGE/SKILLS/ABILITIES REQUIRED
* Minimum level of education and years of relevant work experience.

  • Ph.D. in scientific discipline preferably Analytical Chemistry, Biochemistry, or Systems Biology with 1-2 years’ post-doctoral or Industry experience. Fresh PhD graduates welcome to apply!
* Special knowledge or skills needed and/or licenses or certificates required.

  • Extensive hands-on experience using mass spectrometry instrumentation (e.g. Sciex, Bruker, etc.) with various mass analyzers (i.e. Q-IT, Q-ToF, etc.) for protein analysis.
  • Experience with protein isolation and enrichment strategies from cells/tissues/biofluids to interrogate molecular interaction networks of protein targets.
  • Prior use of label-free and/or isotopic labelling methodologies for quantitative proteomics workflows.
  • Expertise in multi-dimensional LC, micro/nano-LC, or affinity chromatography is required.
  • Demonstrate ability to work on complex problems to produce experimental data across one or more projects.
  • Exercises independent judgement within defined procedures and practices.
  • Demonstrates technical proficiency, collaboration with others within a fast-paced, matrixed, team environment consisting of internal and external team members.
  • Possesses detailed and expert knowledge of scientific principles and concepts.
  • Demonstrate ability to work effectively in a multi-disciplinary team environment.
  • Proficiency with Microsoft Office preferably MS Excel and MS PowerPoint or similar.
  • Excellent verbal and written communication and skills.
  • Analytical thinker with excellent problem-solving skills.
  • Demonstrates an understanding of the strategic alignment of his/her work with corporate goal(s) and demonstrates the ability to adapt to changing priorities and deadlines.
  • Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects.
*Special knowledge or skills and/or licenses or certificates preferred.

  • Detailed experience with bioinformatic tools for analysis of LC-MS datasets (e.g. Skyline, MASCOT, etc.) is needed.
  • Previous work in neurodegeneration, mitochondrial dysfunction, or oncology is preferred.
* Travel requirements
Up to 10%
*Physical requirements (only note if the job requires physical capacity beyond general office work)
Office/Laboratory based position
**At PTC Therapeutics we are seeking candidates in the United States who are fully vaccinated against COVID-19 or who will be fully vaccinated by the time they begin the position.
EEO Statement:
PTC Therapeutics is an equal opportunity employer. We welcome applications from all individuals, regardless of race, color, national origin, gender, age, physical characteristics, social origin, disability, religion, family status, pregnancy, sexual orientation, gender identity, gender expression, disability, veteran status or any unlawful criterion under applicable law. We are committed to treating all applicants fairly and avoiding discrimination.